Characterization of Antibody Binding to Cell Surface Antigens Using a Plasma Membrane-Bound Plate Assay

• Published in: Analytical biochemistry Vol. 224; no. 1; pp. 39 - 50
• Main Authors: Vater, C.A., Reid, K., Bartle, L.M., Goldmacher, V.S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 1995
• Subjects: Animals; Antibodies - analysis; Antibodies - metabolism; Antibodies, Monoclonal - immunology; Antigens, CD - metabolism; Antigens, CD19; Antigens, Differentiation, B-Lymphocyte - metabolism; Antigens, Differentiation, T-Lymphocyte - metabolism; Antigens, Surface - metabolism; Binding, Competitive; CD56 Antigen; Cell Membrane - metabolism; Enzyme-Linked Immunosorbent Assay; Humans; Mice

A procedure has been developed for measuring antibody binding to cell surface antigens using an immobilized plasma membrane fraction. In this method, isolated plasma membranes are dried onto wells of a 96-well microtiter plate and incubated with antibodies that recognize a cell surface protein. Boun...