Characterization of Antibody Binding to Cell Surface Antigens Using a Plasma Membrane-Bound Plate Assay

A procedure has been developed for measuring antibody binding to cell surface antigens using an immobilized plasma membrane fraction. In this method, isolated plasma membranes are dried onto wells of a 96-well microtiter plate and incubated with antibodies that recognize a cell surface protein. Boun...

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Published inAnalytical biochemistry Vol. 224; no. 1; pp. 39 - 50
Main Authors Vater, C.A., Reid, K., Bartle, L.M., Goldmacher, V.S.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 1995
Subjects
Animals
Antibodies - analysis
Antibodies - metabolism
Antibodies, Monoclonal - immunology
Antigens, CD - metabolism
Antigens, CD19
Antigens, Differentiation, B-Lymphocyte - metabolism
Antigens, Differentiation, T-Lymphocyte - metabolism
Antigens, Surface - metabolism
Binding, Competitive
CD56 Antigen
Cell Membrane - metabolism
Enzyme-Linked Immunosorbent Assay
Humans
Mice
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Summary:A procedure has been developed for measuring antibody binding to cell surface antigens using an immobilized plasma membrane fraction. In this method, isolated plasma membranes are dried onto wells of a 96-well microtiter plate and incubated with antibodies that recognize a cell surface protein. Bound antibody is detected indirectly using an enzyme-linked or fluorescently tagged second antibody. Alternatively, the primary antibody itself can be labeled and its binding can be detected directly. The assay is simple and fast and provides several advantages over whole cell binding assays currently in widespread use.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1995.1006