Fatty acid composition, enzyme inhibitory effect, antioxidant and anticancer activity of extract from Saponaria prostrata WILLD. subsp. anatolica HEDGE

• Published in: Bioorganic chemistry Vol. 113; p. 105032
• Main Authors: Kadir Erdogan, Mehmet, Halil Gecibesler, Ibrahim, Yapar, Yakup, Gundogdu, Ramazan, Kirici, Mahinur, Behcet, Lutfi, Taslimi, Parham
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.08.2021
• Subjects: Anticancer; Antioxidant; Caryophyllaceae; Endemic plant; Enzyme inhibition; Enzyme inhibition; Antioxidant; Anticancer; Endemic plant; Caryophyllaceae
• ISSN: 0045-2068; 1090-2120
• DOI: 10.1016/j.bioorg.2021.105032

[Display omitted] •This study attempts to evaluate the antioxidant effect of endemic Saponaria prostrata WILLD. subsp. anatolica HEDGE.•This study attempts to evaluate the enzyme inhibitory of this plant.•This study attempts to evaluate the anticancer properties as well as fatty acid compositions of this plant. This study attempts to evaluate the antioxidant, enzyme inhibitory, and anticancer properties as well as fatty acid compositions of endemic Saponaria prostrata WILLD. subsp. anatolica HEDGE. The gas chromatography-mass spectrometry (GC–MS) was used to determine the fatty acid content of methanol: dichloromethane extract from S. prostrata subsp. anatolica (SPA). Enzymatic activity was measured against acetylcholinesterase, butyrylcholinesterase and α-glucosidase. DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity and Ferric reducing antioxidant power assay (FRAP) were conducted to antioxidant properties. The anticancer effect of SPA on human MCF-7 breast cancer and human HCT116 colorectal cancer cell line was evaluated by WST-1 cell viability assay, colony formation assay and wound healing assay. In addition, human VEGF Elisa method was used to determine the anti-angiogenic effect, and the quantitative real-time PCR (qRT-PCR) method on p53, Bax and Bcl-2 mRNA levels were used to evaluate apoptosis. While high amounts of palmitic acid (40.8%), linoleic acid (17.75%) and α-linolenic acid (16.84%) were detected in the SPA, the total amount of unsaturated fatty acid (51.34%) was higher than the total amount of saturated fatty acid (48.66%). SPA displayed the most promising acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) and α-glycosidase (AG) inhibitory activities (AChE: IC50: 18.03 µg/mL, BuChE: IC50: 44.24 µg/mL and AG: IC50: 210.85 µg/mL). The half maximum inhibitory concentration (IC50) of SPA in MCF-7 and HCT116 cells was determined as 259.79 µg/mL and 97.24 µg/mL, respectively. In addition, it was determined that SPA suppresses colony formation and wound closure, and suppresses angiogenesis as well as triggering apoptosis at a significant level. It is true that endemic S. prostrata subsp. anatolica is a potential source of functional food ingredients, but more analytical and in vivo experiments are needed to explore further secondary metabolite diversity and pharmacological properties.