Total Synthesis of a Miniferredoxin
A miniferredoxin has been designed based on theDesulfovibrio gigasferredoxin II structure and has been successfully synthesized. The 31 amino acid apoprotein was synthesized via standard Fmoc solid phase peptide synthesis andin vitrocluster insertion carried out. The UV-visible spectrum of the minif...
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| Published in | Biochemical and biophysical research communications Vol. 223; no. 2; pp. 360 - 364 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
14.06.1996
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| Subjects | |
| Online Access | Get full text |
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| Abstract | A miniferredoxin has been designed based on theDesulfovibrio gigasferredoxin II structure and has been successfully synthesized. The 31 amino acid apoprotein was synthesized via standard Fmoc solid phase peptide synthesis andin vitrocluster insertion carried out. The UV-visible spectrum of the miniferredoxin (peak at 300 nm and a shoulder at 405 nm) shows the same features as that of theD. gigasferredoxin. Cyclic voltammetry indicated a quasireversible electrode process with a midpoint potential of −370mV vs NHE, which demonstrates that the miniferredoxin is redox active. From these and EPR studies, we propose the incorporation of a Fe4S4cluster. |
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| AbstractList | A miniferredoxin has been designed based on the Desulfovibrio gigas ferredoxin II structure and has been successfully synthesized. The 31 amino acid apoprotein was synthesized via standard Fmoc solid phase peptide synthesis and in vitro cluster insertion carried out. The UV-visible spectrum of the miniferredoxin (peak at 300 nm and a shoulder at 405 nm) shows the same features as that of the D. gigas ferredoxin. Cyclic voltammetry indicated a quasireversible electrode process with a midpoint potential of -370mV vs NHE, which demonstrates that the miniferredoxin is redox active. From these and EPR studies, we propose the incorporation of a Fe4S4 cluster. A miniferredoxin has been designed based on theDesulfovibrio gigasferredoxin II structure and has been successfully synthesized. The 31 amino acid apoprotein was synthesized via standard Fmoc solid phase peptide synthesis andin vitrocluster insertion carried out. The UV-visible spectrum of the miniferredoxin (peak at 300 nm and a shoulder at 405 nm) shows the same features as that of theD. gigasferredoxin. Cyclic voltammetry indicated a quasireversible electrode process with a midpoint potential of −370mV vs NHE, which demonstrates that the miniferredoxin is redox active. From these and EPR studies, we propose the incorporation of a Fe4S4cluster. |
| Author | Christensen, Hans E.M. Sow, Tsin-Chun Pedersen, Marianne V. Ooi, Bee-Lean |
| Author_xml | – sequence: 1 givenname: Tsin-Chun surname: Sow fullname: Sow, Tsin-Chun organization: Department of Chemistry, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260 – sequence: 2 givenname: Marianne V. surname: Pedersen fullname: Pedersen, Marianne V. organization: Chemistry Department A, Technical University of Denmark, 2800, Lyngby, Denmark – sequence: 3 givenname: Hans E.M. surname: Christensen fullname: Christensen, Hans E.M. organization: Institute of Molecular Agrobiology, National University of Singapore, 1 Science Park Drive, Singapore, 118240 – sequence: 4 givenname: Bee-Lean surname: Ooi fullname: Ooi, Bee-Lean organization: Department of Chemistry, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260 |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/8670287$$D View this record in MEDLINE/PubMed |
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| Copyright | 1996 Academic Press |
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| Snippet | A miniferredoxin has been designed based on theDesulfovibrio gigasferredoxin II structure and has been successfully synthesized. The 31 amino acid apoprotein... A miniferredoxin has been designed based on the Desulfovibrio gigas ferredoxin II structure and has been successfully synthesized. The 31 amino acid apoprotein... |
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| StartPage | 360 |
| SubjectTerms | Amino Acid Sequence Anaerobiosis Desulfovibrio - metabolism Electron Spin Resonance Spectroscopy Ferredoxins - chemical synthesis Ferredoxins - chemistry Magnetic Resonance Spectroscopy Molecular Sequence Data Peptides - chemical synthesis Peptides - chemistry Potentiometry Protein Structure, Secondary |
| Title | Total Synthesis of a Miniferredoxin |
| URI | https://dx.doi.org/10.1006/bbrc.1996.0899 https://www.ncbi.nlm.nih.gov/pubmed/8670287 https://search.proquest.com/docview/78110539 |
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