Total Synthesis of a Miniferredoxin

A miniferredoxin has been designed based on theDesulfovibrio gigasferredoxin II structure and has been successfully synthesized. The 31 amino acid apoprotein was synthesized via standard Fmoc solid phase peptide synthesis andin vitrocluster insertion carried out. The UV-visible spectrum of the minif...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 223; no. 2; pp. 360 - 364
Main Authors Sow, Tsin-Chun, Pedersen, Marianne V., Christensen, Hans E.M., Ooi, Bee-Lean
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 14.06.1996
Subjects
Amino Acid Sequence
Anaerobiosis
Desulfovibrio - metabolism
Electron Spin Resonance Spectroscopy
Ferredoxins - chemical synthesis
Ferredoxins - chemistry
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Peptides - chemical synthesis
Peptides - chemistry
Potentiometry
Protein Structure, Secondary
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Summary:A miniferredoxin has been designed based on theDesulfovibrio gigasferredoxin II structure and has been successfully synthesized. The 31 amino acid apoprotein was synthesized via standard Fmoc solid phase peptide synthesis andin vitrocluster insertion carried out. The UV-visible spectrum of the miniferredoxin (peak at 300 nm and a shoulder at 405 nm) shows the same features as that of theD. gigasferredoxin. Cyclic voltammetry indicated a quasireversible electrode process with a midpoint potential of −370mV vs NHE, which demonstrates that the miniferredoxin is redox active. From these and EPR studies, we propose the incorporation of a Fe4S4cluster.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0899