Genome and transcriptome analysis of a newly isolated azo dye degrading thermophilic strain Anoxybacillus sp

• Published in: Ecotoxicology and environmental safety Vol. 203; p. 111047
• Main Authors: Chen, Guotao, An, Xuejiao, Feng, Linlin, Xia, Xiang, Zhang, Qinghua
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 15.10.2020
• Subjects: Anoxybacillus sp; Azo dye; Biodegradation genes; Transcriptome; Whole genome sequence; Biodegradation genes; Whole genome sequence; Anoxybacillus sp; Azo dye; Transcriptome
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2020.111047

Understanding azo dye degrading enzymes and the encoding of their functional genes is crucial for the elucidation of their molecular mechanisms. In this study, a thermophilic strain capable of degrading azo dye was isolated from the soil near a textile dye manufacturing factory. Based on its morphological, physiological and biochemical properties, as well as 16S rRNA gene sequence analysis, the strain was identified as Anoxybacillus sp. PDR2. The decolorization ratios of 100–600 mg/L Direct Black G (DBG) by strain PDR2 reached 82.12–98.39% within 48 h of dyes. Genome analysis revealed that strain PDR2 contains a circular chromosome of 3791144 bp with a G + C content of 42.48%. The genetic basis of azo dye degradation by strain PDR2 and its capacity to adapt to harsh environments, were further elucidated through bioinformatics analysis. RNA-Seq and qRT-PCR technology confirmed that NAD(P)H-flavin reductase, 2Fe-2S ferredoxin and NAD(P)-dependent ethanol dehydrogenase genes expressed by strain PDR2, were the key genes involved in DBG degradation. The combination of genome and transcriptome analysis was utilized to explore the key genes of strain PDR2 involved in azo dye biodegradation, with these findings providing a valuable theoretical basis for the practical treatment of azo dye wastewater. [Display omitted] •A thermophilic Anoxybacillus sp. PDR2 that can effectively degrade DBG was isolated.•The genetic basis of efficient DBG degradation was established by genomic analysis.•RNA-Seq and qRT-PCR technology confirmed the key genes involved in DBG degradation.•DBG is degraded by Anoxybacillus sp. PDR2 via an electron transfer mechanism.