Differential expression and regulation of expansin gene family members during fruit growth and development of 'Shijia' longan fruit

• Published in: Plant growth regulation Vol. 58; no. 3; pp. 225 - 233
• Main Authors: Xie, Hui, Chen, Jian-ye, Yuan, Rong-cai, Zhong, Yu-xiong, Feng, Hai-ling, Xu, Shi-juan, Li, Jian-guo, Lu, Wang-jin
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.07.2009; Springer Netherlands; Springer Nature B.V
• Subjects: Agriculture; Biomedical and Life Sciences; Gene expression; Life Sciences; Original Paper; Plant Anatomy/Development; Plant Physiology; Plant Sciences; Growth and development; Regulation; Expression; Longan fruit; Expansins
• ISSN: 0167-6903; 1573-5087
• DOI: 10.1007/s10725-009-9370-3

In this work, five expansin cDNAs (DlExp1-5) from 'Shijia' longan fruit were isolated and characterized. Moreover, the expression profiles of five expansin genes and the effect of naphthalene acetic acid (NAA) and thidiazuron (TDZ) on their expressions were investigated. The results showed that five expansins exhibited different expression patterns during fruit growth and development. DlExp1 was constitutively expressed in the pericarp while the levels of DlExp1 mRNA in the aril were very high at early stage of fruit development, and decreased gradually from 28 to 77 days after anthesis (DAA). DlExp2 and DlExp4 were related to the growth of pericarp, whereas the expression of DlExp2 and DlExp5 in the aril decreased from 28 to 77 DAA. In addition, NAA and TDZ applied at the stage of rapid pericarp (21 DAA) or aril growth (56 DAA) increased the accumulations of DlExp1 and DlExp2 mRNA in the pericarp and aril, while NAA and TDZ had no or little effect on the accumulations of DlExp3, DlExp4 and DlExp5. DlExp1 and DlExp2 also accumulated highly in rapidly growing tissues, such as young stems and leaves. These findings indicated that Exp genes played a different role in longan fruit growth and showed different response to plant growth substances.