Surface modification of polyvinyl alcohol/malonic acid nanofibers by gaseous dielectric barrier discharge plasma for glucose oxidase immobilization

• Published in: Applied surface science Vol. 385; pp. 349 - 355
• Main Authors: Afshari, Esmail, Mazinani, Saeedeh, Ranaei-Siadat, Seyed-Omid, Ghomi, Hamid
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2016
• Subjects: Air plasma; Dielectric barrier discharge; Electrospinning; Enzymes; Glucose oxidase; Immobilization; Nanofibers; Polyvinyl alcohol; Polyvinyl alcohols; Scanning electron microscopy; Electrospinning; Glucose oxidase; Polyvinyl alcohol; Air plasma; Dielectric barrier discharge; Nanofibers
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2016.05.119

[Display omitted] •We fabricated polyvinyl alcohol/malonic acid nanofibers using electrospinning.•The surface nanofibers were modified by gaseous (air, nitrogen, CO2 and argon) dielectric barrier discharge.•Among them, air plasma had the most significant effect on glucose oxidase immobilization.•Chemical analysis showed that after modification of nanofibers by air plasma, the carboxyl group increased.•After air plasma treatment, reusability and storage stability of glucose oxidase immobilized on nanofibers improved. Polymeric nanofiber prepares a suitable situation for enzyme immobilization for variety of applications. In this research, we have fabricated polyvinyl alcohol (PVA)/malonic acid nanofibers using electrospinning. After fabrication of nanofibers, the effect of air, nitrogen, CO2, and argon DBD (dielectric barrier discharge) plasmas on PVA/malonic acid nanofibers were analysed. Among them, air plasma had the most significant effect on glucose oxidase (GOx) immobilization. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrum analysis and X-ray photoelectron spectroscopy (XPS) results revealed that in case of air plasma modified nanofibers, the carboxyl groups on the surface are increased. The scanning electron microscopy (SEM) images showed that, after GOx immobilization, the modified nanofibers with plasma has retained its nanofiber structure. Finally, we analysed reusability and storage stability of GOx immobilized on plasma modified and unmodified nanofibers. The results were more satisfactory for modified nanofibers with respect to unmodified ones.