Oncolytic avian reovirus σA‐modulated fatty acid metabolism through the PSMB6/Akt/SREBP1/acetyl-CoA carboxylase pathway to increase energy production for virus replication

• Published in: Veterinary microbiology Vol. 273; p. 109545
• Main Authors: Hsu, Chao-Yu, Chen, Yun-Han, Huang, Wei-Ru, Huang, Jing-Wen, Chen, I.-Chun, Chang, Yu-Kang, Wang, Chi-Young, Chang, Ching-Dong, Liao, Tsai-Ling, Nielsen, Brent L., Liu, Hung-Jen
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2022
• Subjects: Acetyl-coA carboxylase α; Acetyl-coA carboxylase β; Ateams; Fatty acids metabolism; Oncolytic avian reovirus; Proteasome 20 S subunit beta 6; Sterol regulatory element-binding protein 1; σA protein; Proteasome 20 S subunit beta 6; ARV; MOI; Ateams; ACC1; Oncolytic avian reovirus; Fatty acids metabolism; ACC2; NLS; MEM; Sterol regulatory element-binding protein 1; CPT1; FBS; HEPES; FAS; Acetyl-coA carboxylase α; SREBP1; SCD1; Acetyl-coA carboxylase β; mTORC1; Vero cells; σA protein
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2022.109545

We have demonstrated previously that the σA protein of avian reovirus (ARV) functions as an activator of cellular energy, which upregulates glycolysis and the TCA cycle for virus replication. To date, there is no report with respect to σA-modulated regulation of cellular fatty acid metabolism. This study reveals that the σA protein of ARV inhibits fatty acids synthesis and enhance fatty acid oxidation by upregulating PSMB6, which suppresses Akt, sterol regulatory element-binding protein 1 (SREBP1), acetyl-coA carboxylase α (ACC1), and acetyl-coA carboxylase β (ACC2). SREBP1 is a transcription factor involved in fatty acid and cholesterol biosynthesis. Overexpression of SREBP1 reversed σA-modulated suppression of ACC1 and ACC2. In this work, a fluorescence resonance energy transfer‐based genetically encoded indicator, Ateams, was used to study σA-modulated inhibition of fatty acids synthesis which enhances cellular ATP levels in Vero cells and human cancer cell lines (A549 and HeLa). By using Ateams, we demonstrated that σA-modulated inhibition of Akt, SREBP1, ACC1, and ACC2 leads to increased levels of ATP in mammalian and human cancer cells. Furthermore, knockdown of PSMB6 or overexpression of SREBP1 reversed σA-modulated increased levels of ATP in cells, indicating that PSMB6 and SREBP1 play important roles in ARV σA-modulated cellular fatty acid metabolism. Furthermore, we found that σA R155/273A mutant protein loses its ability to enter the nucleolus, which impairs its ability to regulate fatty acid metabolism and does not increase ATP formation, suggesting that nucleolus entry of σA is critical for regulating cellular fatty acid metabolism to generate more energy for virus replication. Collectively, this study provides novel insights into σA-modulated inhibition of fatty acid synthesis and enhancement of fatty acid oxidation to produce more energy for virus replication through the PSMB6/Akt/SREBP1/ACC pathway. •σA-modulated inhibition of fatty acid synthesis and enhancement of fatty acid oxidation to produce more energy for virus replication.•By using Ateams, in situ detection of σA-modulated increase in ATP levels in cells can be performed.•σA R155/273A mutant protein loses its ability to enter the nucleolus, which does not increase ATP formation.