Effects of permanent magnetic fields on the proliferation of Phalaenopsis protocorm-like bodies using liquid medium

• Published in: Scientia horticulturae Vol. 128; no. 4; pp. 479 - 484
• Main Authors: Van, Pham Thanh, Teixeira da Silva, Jaime A., Ham, Le Huy, Tanaka, Michio
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.05.2011; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Culture media; Culture vessel; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Magnetic fields; Micropropagation; Miracle Pack; Phalaenopsis; Protocorm-like body; Vegetative propagation. Micropropagation; Vegetative propagation. Sowing and planting. Harvesting; Culture vessel; Culture media; Phalaenopsis; Micropropagation; Magnetic fields; Protocorm-like body; Miracle Pack; Culture medium; Monocotyledones; Horticulture; Plant juvenile growth stage; Ornamental crop; Liquid medium; Proliferation; Organogenesis; Vessel; Orchidaceae; Angiospermae; Spermatophyta; Magnetic field
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2011.01.018

► This is the first study ever to address the effects of magnetic fields (MFs) on the proliferation of Phalaenopsis protocorm-like bodies (PLBs). ► This is also the first experiment about the proliferation of Phalaenopsis PLBs using liquid medium in the “Miracle Pack ®” culture system. ► The findings of our study are clear, i.e., there are actual effects on the proliferation of PLBs: South pole had a stronger effect than the North pole. ► The MF of 0.1 T – S resulted in the greatest fresh and dry weights of regenerated PLBs. Magnetic fields (MFs) have been applied for the first time in orchid micropropagation. Protocorm-like bodies (PLBs) – approximately 3 mm in diameter – first derived from leaf segment culture of Phalaenopsis Gallant Beau ‘George Vazquez’, were subcultured every 2 months, and served as initial explants. The proliferation of Phalaenopsis PLBs in liquid medium in the Miracle Pack ® culture system was affected by the action of different intensities and polarities of MFs: 0.1, 0.15 and 0.2 Tesla (T) at North (N) and South (S) poles. The MF of 0.1 T – S resulted in the greatest fresh weight of regenerated PLBs. The average number of neo-PLBs formed per clusters in the PLB treated by MF: 0.1–0.2 T was decreased compared to the control exposed to natural MF (5 × 10 −6 T). The proliferation of PLBs under 0.15 T – MF at both N and S poles for 2 and 7 weeks demonstrated that a longer duration of exposure to an MF of 0.15 T, regardless of the polarity, resulted in greater biomass of newly formed PLBs and smaller average number of newly formed PLBs. The S pole of MF had stronger effects on Phalaenopsis PLBs proliferation than the N pole did in all treatments.