Isolation and characterization of microsatellite markers for the clam Ruditapes philippinarum and cross-species amplification with the clam Ruditapes variegate

• Published in: Conservation genetics Vol. 10; no. 6; pp. 1821 - 1823
• Main Authors: An, Hye Suck, Kim, Eun Mi, Park, Jung Youn
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.12.2009; Springer Netherlands; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Biodiversity; Biomedical and Life Sciences; Conservation; Conservation Biology/Ecology; Conservation genetics; Ecology; Evolutionary Biology; Fisheries management; Fishery resources; Genetic diversity; Genetic resources; Genetics; Life Sciences; Mollusks; Plant Genetics and Genomics; Population genetics; Shellfish; Sustainability management; Sustainable development; Technical Note; South Korea; Cross-amplification; Genetic markers; Microsatellites
• ISSN: 1566-0621; 1572-9737
• DOI: 10.1007/s10592-009-9824-1

Ruditapes philippinarum and R. variegate are commercially important shellfish in Korea. In order to understand the processes and organization of genetic diversity and genetic resources for the sustainable management of fisheries resources we developed 13 primer pairs for microsatellite loci in R. philippinarum and tested their cross-amplification in R. variegate. Twelve primers amplified in both species. Nine loci were polymorphic in R. philippinarum and eight in R. variegate, each with nine to 26 alleles per locus. Unbiased expected heterozygosity levels varied from 0.73 to 0.94 in R. philippinarum. All polymorphic loci possessed species-specific alleles. No linkage disequilibrium was found. Results indicated that these microsatellite were highly polymorphic and will be useful for conservation genetics of both species.