A Novel Cl −-Pump: Intracellular Regulation of Transport Activity

Utilizing a purified basolateral plasma membrane vesicle preparation containing Cl −-ATPase, it was demonstrated that ATP, and its subsequent hydrolysis, stimulated both intravesicular Cl − accumulation and intravesicular negativity with almost identical kinetics. Mg 2+ also stimulated both pump tra...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 215; no. 3; pp. 994 - 1000
Main Authors Gerencser, G.A., Purushotham, K.R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 24.10.1995
Subjects
Adenosine Triphosphatases - metabolism
Adenosine Triphosphate - metabolism
Animals
Anion Transport Proteins
Aplysia
Biological Transport
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology
Cations, Divalent - pharmacology
Cell Fractionation
Cell Membrane - enzymology
Cell Membrane - physiology
Centrifugation, Density Gradient
Chlorides - metabolism
Epithelium - enzymology
Kinetics
Magnesium - pharmacology
Membrane Potentials
Vanadates - pharmacology
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Summary:Utilizing a purified basolateral plasma membrane vesicle preparation containing Cl −-ATPase, it was demonstrated that ATP, and its subsequent hydrolysis, stimulated both intravesicular Cl − accumulation and intravesicular negativity with almost identical kinetics. Mg 2+ also stimulated both pump transport characteristics with an optimal concentration of 3 mM. Similarly, the pH optimum for both pump transport expressions was 7.8. Orthovanadate inhibition of both pump transport characteristics was directly related to its concentration. These results suggested that the active Cl − transport mechanism is electrogenic and is driven by a Cl −-stimulated ATPase.
Bibliography:ObjectType-Article-1
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2562