Interaction between polyamines and ethylene in the response to salicylic acid under normal photoperiod and prolonged darkness

• Published in: Plant physiology and biochemistry Vol. 167; pp. 470 - 480
• Main Authors: Takács, Zoltán, Poór, Péter, Tari, Irma
• Format: Journal Article
• Language: English
• Published: Elsevier Masson SAS 01.10.2021
• Subjects: Ethylene; Illuminated and dark samples; Never ripe mutant; Polyamines; Salicylic acid; Tomato; Salicylic acid; Illuminated and dark samples; Polyamines; Tomato; Ethylene; Never ripe mutant
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2021.08.009

The impact of salicylic acid (SA) on ethylene (ET) production and polyamine (PA) metabolism was investigated in wild type (WT) and ET receptor mutant Never ripe (Nr) tomato leaves under normal photoperiod and prolonged darkness. Nr displayed higher ET emanation compared to WT under control conditions and after SA treatments, but the ET signalling was blocked in these tissues. The accumulation of PAs was induced by 1 mM but not by 0.1 mM SA and was higher in WT than in Nr leaves. Upon 1 mM SA treatment, which caused hypersensitive response, illuminated leaves of WT showed high spermine (Spm) content in parallel with an increased expression of S-adenosylmethionine decarboxylase and Spm synthase (SlSPMS) suggesting that this process depended on the light. In Nr, however, Spm content and the expression of the SlSPMS gene were very low independently of the light conditions and SA treatments. This suggests that Spm synthesis needs functional ET perception. In WT leaves 1 mM SA enhanced putrescine (Put) synthesis by increasing the expression of Put biosynthesis genes, arginine and ornithine decarboxylases under darkness, while they were down-regulated in Nr. The activities of diamine (DAO) and polyamine oxidases (PAO), however, were generally higher in Nr compared to the WT after SA treatments. In Nr both SA applications increased the expression of SlPAO1 under normal photoperiod, while SlPAO2 was down-regulated in the dark suggesting a diverse role of PAOs in PA catabolism. These results indicated that ET could modulate the SA-induced PA metabolism in light-dependent manner. •Salicylic acid (SA)-induced polyamine metabolism is controlled by ethylene and light.•SA-induced spermine synthesis and accumulation were enhanced in the light in WT.•Spermine accumulation and the expression of SlSPMS gene require ethylene signalling.•Putrescine and its biosynthesis induced by salicylic acid are dominant in the dark.