Glutaredoxin like protein (RtGRL1) regulates H2O2 and Na+ accumulation by maintaining the glutathione pool during abiotic stress

• Published in: Plant physiology and biochemistry Vol. 159; pp. 135 - 147
• Main Authors: Ma, Binjie, Suo, Yafei, Zhang, Jie, Xing, Ningning, Gao, Ziqi, Lin, Xiaofei, Zheng, Linlin, Wang, Yingchun
• Format: Journal Article
• Language: English
• Published: Elsevier Masson SAS 01.02.2021
• Subjects: Abiotic stress; Glutaredoxin like protein; Glutathione; Reaumuria trigyna; ROS; Glutaredoxin like protein; Reaumuria trigyna; Abiotic stress; ROS; Glutathione
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2020.11.040

Reaumuria trigyna, an endangered recretohalophyte, is a small archaic wild shrub endemic to arid and semiarid plateau regions of Inner Mongolia, China. Based on salt-related transcriptomic data, we isolated a GRX family gene, glutaredoxin like protein (RtGRL1), from R. trigyna that is associated with the removal of active oxygen and regulation of redox status. RtGRL1 encodes a plasma membrane and chloroplast-localized protein induced by salt, cold, drought stress, ABA, and H2O2. In Arabidopsis thaliana, ectopically expressed RtGRL1 positively regulated biomass accumulation, chlorophyll content, germination rate, and primary root length under salt and drought stress. Overexpression of RtGRL1 induced expression of genes related to antioxidant enzymes and proline biosynthesis, thus increasing glutathione biosynthesis, glutathione-dependent detoxification of reactive oxygen species (ROS), and proline content under stress. Changes in RtGRL1 expression consistently affected glutathione/oxidizedglutathione and ascorbate/dehydroascorbate ratios and H2O2 concentrations. Furthermore, RtGRL1 promoted several GSH biosynthesis gene transcripts, decreased leaf Na+ content, and maintained lower Na+/K+ ratios in transgenic A. thaliana compared to wild type plants. These results suggest a critical link between RtGRL1 and ROS modulation, and contribute to a better understanding of the mechanisms governing plant responses to drought and salt stress. •Overexpression of RtGRL1 induced expression of genes related increasing glutathione biosynthesis and proline content under abiotic stress.•RtGRL1 improved plant ability of glutathione-dependent detoxification of reactive oxygen species (H2O2).•RtGRL1 maintained glutathione pool balance and decreased leaf Na+ content, and increased water retention capacity.