Interleukin-1β Activates PI 3-Kinase in Renal Mesangial Cells

Interleukin-1β rapidly stimulates the activation of phosphatidylinositol 3-hydroxyl kinase in renal mesangial cells. This interleukin-1β dependent activation is associated with a rapid increase in phosphatidyl inositol 3,4,5 phosphate in renal mesangial cells. The increase in PI 3-kinase activity is...

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Published inBiochemical and biophysical research communications Vol. 227; no. 1; pp. 289 - 293
Main Authors Donaldson, Audrey, Daphna-Iken, Dorit, Tetsuka, Toshifumi, Morrison, Aubrey R.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 03.10.1996
Subjects
Androstadienes - pharmacology
Animals
Cells, Cultured
Dinoprostone - biosynthesis
Enzyme Activation
Enzyme Inhibitors - pharmacology
Glomerular Mesangium - cytology
Glomerular Mesangium - enzymology
Interleukin-1 - antagonists & inhibitors
Interleukin-1 - pharmacology
Male
Nitric Oxide - biosynthesis
Phosphatidylinositol 3-Kinases
Phosphotransferases (Alcohol Group Acceptor) - metabolism
Rats
Rats, Sprague-Dawley
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Summary:Interleukin-1β rapidly stimulates the activation of phosphatidylinositol 3-hydroxyl kinase in renal mesangial cells. This interleukin-1β dependent activation is associated with a rapid increase in phosphatidyl inositol 3,4,5 phosphate in renal mesangial cells. The increase in PI 3-kinase activity is inhibited by wortmannin. In addition wortmannin partially inhibited IL-1β induced PGE2production and potentiated IL-1β induced nitric oxide production. These experiments suggest that IL-1β can activate PI 3-kinase in renal mesangial cells and that the enzyme plays a role in IL-1β induced PGE2and NO formation in the renal mesangial cell.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.1502