Overexpression of Ginkgo BBX25 enhances salt tolerance in Transgenic Populus

• Published in: Plant physiology and biochemistry Vol. 167; pp. 946 - 954
• Main Authors: Huang, Shujing, Chen, Caihui, Xu, Mengxuan, Wang, Guibin, Xu, Li-an, Wu, Yaqiong
• Format: Journal Article
• Language: English
• Published: Elsevier Masson SAS 01.10.2021
• Subjects: Abiotic stress; BBX; Ginkgo; Overexpression; Transgenic Populus; BBX; Overexpression; Ginkgo; Abiotic stress; Transgenic Populus
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2021.09.021

B-box (BBX) genes play important roles in plant growth, light morphogenesis, and environmental stress responses. Ginkgo (Ginkgo biloba L.) is known as a living fossil species that has a strong ability to adapt to environmental changes and tolerate harsh conditions. In this study, we chose this species to investigate the function of the GbBBX25 gene. We isolated the BBX gene from ginkgo and named it GbBBX25; this gene consists of an 819 bp open reading frame (ORF) that encodes 273 amino acids with two B-box domains but no CCT domain. GbBBX25 was localized in only the nucleus. The expression of GbBBX25 transcripts was observed in the leaves and was significantly enhanced under salt stress conditions. To further verify its function, we overexpressed the GbBBX25 gene in Populus davidiana × Populus bolleana and found that the transgenic Populus had greater soluble sugar levels and higher peroxidase (POD) activity in response to salt stress than nontransgenic (NT) Populus. Five genes related to salt stress were induced in transgenic plants with significantly higher expression levels than those in NT plants. This finding suggests that GbBBX25 improves the salt adaptation abilities of transgenic Populus and provides a scientific basis for related research. •The greatest expression of GbBBX25 was observed in the leaves and under salt stress.•By enhancing the antioxidant defense, GbBBX25 may improve salt-adaptation abilities.•Increasing GbBBX25 expression can potentially be used in breeding to counteract abiotic stress.