Polymorphic microsatellites in the Reeves's pheasant developed by cross-species amplification

Cross-species microsatellite amplification is an effective way of obtaining microsatellite loci for closely related taxa in bird species. The Reeves's pheasant, Syrmaticus reevesii, is a vulnerable species endemic to China. To improve population genetics and parentage analysis studies in this s...

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Bibliographic Details
Published inEuropean journal of wildlife research Vol. 55; no. 6; pp. 627 - 629
Main Authors Wang, Ning, Chang, Jiang, Gu, Lang-Yu, Zhang, Zheng-Wang
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.12.2009
Springer-Verlag
Springer Nature B.V
Subjects
Animal populations
Biomedical and Life Sciences
Ecology
Endemic species
Fish & Wildlife Biology & Management
Genetic diversity
Genetic markers
Genetics
Genotype & phenotype
Life Sciences
Population genetics
Short Communication
Threatened species
Wildfowl
Zoology
Cross-species amplification
Microsatellites
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Summary:Cross-species microsatellite amplification is an effective way of obtaining microsatellite loci for closely related taxa in bird species. The Reeves's pheasant, Syrmaticus reevesii, is a vulnerable species endemic to China. To improve population genetics and parentage analysis studies in this species, we obtained nine polymorphic microsatellite markers, in addition to the nine markers previously isolated, from the cross-species amplification of 52 markers. The number of alleles per locus varied between two and 12 with expected heterozygosity ranging from 0.298 to 0.714 (n = 107). The success rates of simulated paternity tests using CERVUS software improved at different confidence levels after adding these markers to the previous ones.
Bibliography:http://dx.doi.org/10.1007/s10344-009-0307-y
ISSN:1612-4642
1439-0574
DOI:10.1007/s10344-009-0307-y