Analysis of phenolic compounds in Greek plants of Lamiaceae family by HPLC

• Published in: Journal of applied research on medicinal and aromatic plants Vol. 6; pp. 62 - 69
• Main Authors: Skendi, Adriana, Irakli, Maria, Chatzopoulou, Paschalina
• Format: Journal Article
• Language: English
• Published: Elsevier GmbH 01.09.2017
• Subjects: Antioxidant activity; applied research; Aromatic medicinal plants; carvacrol; Coridothymus capitatus; detection limit; Diode array detection; essential oil crops; high performance liquid chromatography; HPLC; Lamiaceae family; Melissa officinalis; Origanum vulgare subsp. hirtum; Phenolics; Rosmarinus officinalis; Satureja; thymol; SRA; PRCA; TPC; Phenolics; SA; Lamiaceae family; Diode array detection; GA; FRAP; 4-HBA; CA; HPLC; QUE; THY; DPPH; DAD; Antioxidant activity; MYR; RMA; CIN; EPIG; LUT; GAE; VA; PCA; RUT; RP-HPLC; TE; CAR; KAM; CAT; CLA; API; FA; NAR; ABTS; Aromatic medicinal plants; EPI
• ISSN: 2214-7861; 2214-7861
• DOI: 10.1016/j.jarmap.2017.02.001

[Display omitted] •HPLC-DAD method was developed and validated for 24 phenolics.•Validation results exhibited that this method was accurate and selective.•The method is ideally suited for routine analysis in plants of Lamiaceae family.•Rosmarinic acid is the main phenolic in lemon balm and rosemary extracts.•Carvacrol is the major phenolic in Greek oregano, thymus and satureja. The objective of the current study is the simultaneous determination of 24 phenolics including carvacrol and thymol, in plants of Lamiaceae family by developing a simple and reliable reversed-phase high-performance liquid chromatographic method involving diode array detection. The separation of 24 compounds was achieved on a reversed phase C18 column kept at 30°C by gradient elution with a flow rate of 1.3mL/min. The method was validated for specificity, linearity and intra- and inter-day precision. The limit of detection and quantification for all analytes were small, ranging from 0.002 to 0.16 and 0.01 to 0.48μg/mL, respectively. This method was successfully applied to detect and quantify phenolics present in the plants. It was found that the amount of phenolics in both extracts (water, and methanolic) decreased in the following order: Origanum vulgare ssp. hirtum>Thymus capitatus>Satureja thymbra>Melissa officinalis>Rosmarinus officinalis. The limit of quantification values are low enough to detect and quantify successfully small amounts of 24 phenolics in the plants of Lamiaceae family. Generally, Melissa officinalis exhibited the highest antioxidant activity among the samples followed by Origanum vulgare ssp. hirtum. The developed method, is simple and reliable with satisfactory sensitivity and precision and is ideally suited for routine analysis.