The Reaction of Nitroxyl (HNO) with Nitrosobenzene Gives Cupferron (N-Nitrosophenylhydroxylamine)
Nitroxyl (HNO), a penultimate product in the NOS-catalyzed conversion ofl-arginine tol-citrulline, generated from Angeli's salt (AS) was determined by trapping it with nitrosobenzene (NB) to produce cupferron. The cupferron thus produced was characterized by complexation with Fe3+, Al3+, Cu2+,...
Saved in:
Published in | Nitric oxide Vol. 2; no. 1; pp. 66 - 72 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.01.1998
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Nitroxyl (HNO), a penultimate product in the NOS-catalyzed conversion ofl-arginine tol-citrulline, generated from Angeli's salt (AS) was determined by trapping it with nitrosobenzene (NB) to produce cupferron. The cupferron thus produced was characterized by complexation with Fe3+, Al3+, Cu2+, or Sn2+. UV/VIS spectra of the solubilized (in CHCl3) precipitates formed from NB and nitroxyl generated from AS in the presence of the iron, aluminum, copper, or tin salts were identical to those of their corresponding cupferron complexes. The identities of the Fe3+and Cu2+complexes formed from NB and HNO were further confirmed by their identical retention times on HPLC when compared to authentic Fe3+and Cu2+cupferron complexes. It was possible to detect 5 × 10−6M of the cupferron Fe3+complex spectrophotometrically and to measure its production from the nitroxyl generators AS and methanesulfohydroxamic acid (MSHA) in the presence of 10−4M NB. The yield of cupferron was 51 and 62% of the amount of nitroxyl possible from AS or MSHA, respectively, after taking into account the relative rates of nitroxyl generation from these donors. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1089-8603 1089-8611 |
DOI: | 10.1006/niox.1998.0166 |