The Metastasis Suppressor Candidate Nucleotide Diphosphate Kinase NM23 Specifically Interacts with Members of the ROR/RZR Nuclear Orphan Receptor Subfamily

• Published in: Biochemical and biophysical research communications Vol. 227; no. 1; pp. 82 - 87
• Main Authors: Paravicini, G., Steinmayr, M., André, E., Becker-André, M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.10.1996
• Subjects: Animals; Base Sequence; Genes, myc; Humans; Mice; Molecular Sequence Data; Monomeric GTP-Binding Proteins; NM23 Nucleoside Diphosphate Kinases; Nucleoside-Diphosphate Kinase; Protein Binding; Rats; Receptors, Cytoplasmic and Nuclear - metabolism; Sequence Homology, Nucleic Acid; Substrate Specificity; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1996.1471

We have cloned proteins that interact with the nuclear orphan receptor RZRβ using the yeast two-hybrid system. We identified, amongst a number of other genes, the nucleoside diphosphate kinase (NDPK)-2 also known as Nm23-2, c-mycregulatory factor PuF and differentiation inhibitory factor. RZRβ specifically interacts with Nm23-2 but not with the closely related tumor metastasis suppressor candidate gene product Nm23-1. In contrast, RORα interacts with both Nm23 proteins. These findings were corroborated byin vitrointeraction assays based on GST-pulldown experiments. With n-mycwe propose a candidate gene regulated by RORα/RZRβ and Nm23, based on the finding that the respective DNA binding sites in the first intron are conserved in several mammalian species.