Elucidating the impact of in vitro cultivation on Nicotiana tabacum metabolism through combined in silico modeling and multiomics analysis
The systematical characterization and understanding of the metabolic behaviors are the basis of the efficient plant metabolic engineering and synthetic biology. Genome-scale metabolic networks (GSMNs) are indispensable tools for the comprehensive characterization of overall metabolic profile. Here w...
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Published in | Frontiers in plant science Vol. 14; p. 1281348 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
03.11.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The systematical characterization and understanding of the metabolic behaviors are the basis of the efficient plant metabolic engineering and synthetic biology. Genome-scale metabolic networks (GSMNs) are indispensable tools for the comprehensive characterization of overall metabolic profile. Here we first constructed a GSMN of tobacco, which is one of the most widely used plant chassis, and then combined the tobacco GSMN and multiomics analysis to systematically elucidate the impact of
in-vitro
cultivation on the tobacco metabolic network.
In-vitro
cultivation is a widely used technique for plant cultivation, not only in the field of basic research but also for the rapid propagation of valuable horticultural and pharmaceutical plants. However, the systemic effects of
in-vitro
cultivation on overall plant metabolism could easily be overlooked and are still poorly understood. We found that
in-vitro
tobacco showed slower growth, less biomass and suppressed photosynthesis than soil-grown tobacco. Many changes of metabolites and metabolic pathways between
in-vitro
and soil-grown tobacco plants were identified, which notably revealed a significant increase of the amino acids content under
in-vitro
condition. The
in silico
investigation showed that
in-vitro
tobacco downregulated photosynthesis and primary carbon metabolism, while significantly upregulated the GS/GOGAT cycle, as well as producing more energy and less NADH/NADPH to acclimate
in-vitro
growth demands. Altogether, the combination of experimental and
in silico
analyses offers an unprecedented view of tobacco metabolism, with valuable insights into the impact of
in-vitro
cultivation, enabling more efficient utilization of
in-vitro
techniques for plant propagation and metabolic engineering. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2023.1281348 |