Induction of Tyrosine Phosphorylation and Translocation of Ezrin by Hepatocyte Growth Factor/Scatter Factor (HGF/SF)

• Published in: Biochemical and biophysical research communications Vol. 217; no. 3; pp. 1062 - 1069
• Main Authors: Jiang, W.G., Hiscox, S., Singhrao, S.K., Puntis, M.C.A., Nakamura, T., Mansel, R.E., Hallett, M.B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.12.1995
• Subjects: Actins - metabolism; Cell Compartmentation; Cell Membrane - ultrastructure; Cytoskeletal Proteins; Enzyme Inhibitors - pharmacology; Genistein; Hepatocyte Growth Factor - physiology; Humans; Immunohistochemistry; Isoflavones - pharmacology; Phosphoproteins - metabolism; Phosphorylation; Phosphotyrosine - metabolism; Protein-Tyrosine Kinases - antagonists & inhibitors; Tumor Cells, Cultured
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1995.2877

Ezrin is a member of the TERM family and is a key protein in cytoplasmic membrane-cytoskeleton interactions; This study showed that hepatocyte growth factor/scatter factor (HGF/SF), a cytokine known to regulate motility, morphogenesis and growth of cells, stimulated the tyrosine phosphorylation of ezrin in a human colon epithelial cell line, HT115. After HGF/SF stimulation, ezrin translocated from the cytosol and generalised membrane to the areas of ruffled membrane as visualised by indirect immunofluorescent and immunogold electron microscopy. This effect was inhibited by genistein, a tyrosine kinase inhibitor, It is concluded that HGF/SF induces ezrin translocation by stimulation of its tyrosine phosphorylation and that this plays a key role in HGF/SF induced membrane ruffling.