Joint Cultivation of Human Erythroblastoid Cells and Mouse Fibroblasts Triggers Release of a Wide Spectrum of Cytotoxic Factors

It has been demonstrated that joint cultivation of human K-562 cells and mouse L-929 cells results in triggering apoptosis in L-929 cells and possibly also in K-562 cells. Analysis of proteins recovered from the culture medium has revealed the presence of three cytotoxic factors including TNF-α. Thi...

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Published inBiochemical and biophysical research communications Vol. 234; no. 3; pp. 655 - 659
Main Authors Gnuchev, N.V., Sashchenko, L.P., Iarovaia, O.V., Dukhanina, E., Lukianova, T., Razin, S.V.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 29.05.1997
Subjects
Animals
Apoptosis
Cell Line
Coculture Techniques
Culture Media, Conditioned
DNA Fragmentation
Erythroblasts - cytology
Erythroblasts - metabolism
Fibroblasts - cytology
Fibroblasts - metabolism
Humans
Mice
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:It has been demonstrated that joint cultivation of human K-562 cells and mouse L-929 cells results in triggering apoptosis in L-929 cells and possibly also in K-562 cells. Analysis of proteins recovered from the culture medium has revealed the presence of three cytotoxic factors including TNF-α. This is the first observation of a release of cytotoxic factors by non-lymphoid cells in response to a direct contact with cells of different origin.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1997.6617