5-HT1A receptor in the central amygdala and 5-HT2A receptor in the basolateral amygdala are involved in social hierarchy in male mice

• Published in: European journal of pharmacology Vol. 957; p. 176027
• Main Authors: Jiang, Yi, Zhou, Jie, Song, Bai-Lin, Wang, Yan, Zhang, Dong-Lin, Zhang, Zheng-Tian, Li, Lai-Fu, Liu, Ying-Juan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.10.2023
• Subjects: 5-HT receptors; Amygdala; Anxiety; Social hierarchy; Anxiety; Amygdala; Social hierarchy; 5-HT receptors
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2023.176027

Most social animals self-organize into dominance hierarchies that strongly influence their behavior and health. The serotonin (5-HT) system is believed to play an important role in the formation of social hierarchy. 5-HT receptors are abundantly expressed in the amygdala, which is considered as the central node for the perception and learning of social hierarchy. In this study, we assessed the functions of various 5-HT receptor subtypes related to social rank determination in different subregions of the amygdala using the confrontation tube test in mice. We revealed that most adult C57BL/6 J male mice exhibited a linear social rank after a few days of cohousing. The tube test ranks were slightly related to anxiety-like behavioral performance. After the tube test, the amygdala and 5-HT neurons in the dorsal raphe nucleus were activated in lower-rank individuals. Quantitative real-time polymerase chain reaction analysis revealed that despite the high expression of 5-HT1A receptor mRNA in the central amygdala (CeA), 5-HT2A receptor mRNA expression was downregulated in the basolateral amygdala (BLA) in higher-rank individuals. The dominant–subordinate relationship between mouse pairs could be switched via pharmacological modulation of these receptors in CeA and BLA, suggesting that these expression changes are essential for establishing social ranks. Our findings provide novel insights into the divergent functions of 5-HT receptors in the amygdala related to social hierarchy, which is closely related to our health and welfare. •Male mice established a linear social rank after a few days of cohousing.•5-HT neurons in the dorsal raphe nucleus were activated in low-ranking individuals.•5-HT1A receptor were elevated in the CeA of high-ranking individuals.•5-HT2A receptor were down-regulated in the BLA of high-ranking individuals.•Pharmacological modulation of these receptors switched the dominant–subordinate relationship.