A demonstration of human beta-2-microglobulin specific association with the surface of teleostean cells

Purified human Beta-2 microglobulin (ß2m) and a human 2m fluorochrome conjugate were used in exchange reactions to demonstrate that ß2m associates with a teleostean cell surface protein. 2m exchange among brown bullhead, channel catfish, fathead minnow, and rainbow trout cell lines was detected by u...

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Published inDevelopmental and comparative immunology Vol. 18; no. 6; pp. 483 - 494
Main Authors Kunich, John C., Contreras, Bernardo, Recktenwald, Diether J., Nieto, Maria C.
Format Journal Article
LanguageEnglish
Published United States Elsevier Ltd 01.11.1994
Subjects
Animals
beta 2-Microglobulin - metabolism
Beta-2-microglobulin
Binding, Competitive - physiology
Cell Line
Conjugate
Cyanine fluorochrome
Cyprinidae - immunology
Electrophoresis, Polyacrylamide Gel
Fishes - immunology
Flow Cytometry
Fluorescent Antibody Technique
Freshwater
Humans
Ictaluridae - immunology
Ictalurus nebulosus
Ictalurus punctatus
Major Histocompatibility Complex (MHC)
Membrane Proteins - immunology
Oncorhynchus mykiss
Oncorhynchus mykiss - immunology
Pimephales promelas
Protein Binding - immunology
Radioimmunoassay
Teleostean
Teleostei
ß2m exchange
Flow cytometry
Conjugate
radioimmunoassay
Cyanine fluorochrome
Major Histocompatibility Complex (MHC)
Beta-2-microglobulin
Teleostean
ß2m exchange
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Summary:Purified human Beta-2 microglobulin (ß2m) and a human 2m fluorochrome conjugate were used in exchange reactions to demonstrate that ß2m associates with a teleostean cell surface protein. 2m exchange among brown bullhead, channel catfish, fathead minnow, and rainbow trout cell lines was detected by using either radioimmunoassay or flow cytometry. Evidence that 2m binds specifically with the surface of teleostean cells and possibly associates with an expressed class I MHC homologue is provided. Moreover, following exchange on brown bullhead cells, a coprecipitated protein of 45 kDa was observed following subsequent immunoprecipitation with the human ß2m specific antibody B1.1G6. Given that β2m is a peripheral protein which has been shown to exchange with MHC expressing cells from different species, co-precipitation results suggest that the 45 kDa protein may represent a class I MHC homologue.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0145-305X
1879-0089
DOI:10.1016/S0145-305X(06)80003-0