Transformation of Mollicutes with Single-Stranded Tn4001 DNA
Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log l...
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Published in | Plasmid Vol. 32; no. 1; pp. 85 - 88 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.07.1994
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Subjects | |
Online Access | Get full text |
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Summary: | Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log lower frequency than did A. oculi ISM1499. Restriction enzyme digestion of single-stranded DNA indicated homologous base pairing in the inverted repeat regions, which could account for the transpositional activity of single-stranded DNA. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0147-619X 1095-9890 |
DOI: | 10.1006/plas.1994.1048 |