The Effect of Testosterone Replacement Therapy on Bladder Functions, Histology, Apoptosis and Rho-kinase Expression in Bladder Outlet Obstruction and Hypogonadism Rat Model

The effect of testosterone replacement therapy was investigated on bladder functions, histology, apoptosis as well as Rho-kinase expression in the rat bladder outlet obstruction (BOO) and hypogonadism models. 30 mature male rats divided into 4 groups: sham group (n = 8), BOO group (n = 8), BOO + orc...

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Published inTurkish journal of medical sciences Vol. 49; no. 3; pp. 1491 - 1499
Main Authors Saylam, BariŞ, Efesoy, Ozan, BozdoĞan Arpaci, Rabİa, Tİftİk, Rukİye Nalan, Tek, Mesut, BÜyÜkafŞar, Kansu, Bozlu, Murat, Çayan, Selahİttİn
Format Journal Article
LanguageEnglish
Published Turkey The Scientific and Technological Research Council of Turkey 28.06.2021
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Summary:The effect of testosterone replacement therapy was investigated on bladder functions, histology, apoptosis as well as Rho-kinase expression in the rat bladder outlet obstruction (BOO) and hypogonadism models. 30 mature male rats divided into 4 groups: sham group (n = 8), BOO group (n = 8), BOO + orchiectomy group (n = 7), BOO + orchiectomy + testosterone (T) treatment group (n = 7). Cystometric findings, apoptosis index, Rho-kinase (ROCK-2) expression and smooth muscle / collagen ratio were compared. BOO did not change ROCK-2 expression level, compared to sham group (p > 0.05). However, when compared to BOO group (p < 0.01), BOO + orchiectomy led ROCK-2 increase. The testosterone treatment failed to reverse the up-regulation of ROCK-2 induced by orchiectomy although it tended to lower ROCK-2 level. Compared to sham group (p = 0.002), changes in maximal bladder capacity and leak point pressure were higher (p = 0.026, p = 0.001), and bladder compliance was lower in BOO group. Also the apoptosis index was different between the two groups (p = 0.380). Smooth muscle / collagen ratio was higher in BOO + orchiectomy + T group than in BOO + orchiectomy group (p = 0.010). The research draws attention to alternating treatment approaches in case of the presence of hypogonadism and BOO.
Bibliography:The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. The authors received no financial support for the research, authorship, and/or publication of this article.
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ISSN:1300-0144
1303-6165
DOI:10.3906/sag-2004-15