Modification of serological techniques and their evaluation for detection of potato viruses in seed certification related activities

• Published in: Crop protection Vol. 61; pp. 51 - 57
• Main Authors: Samsatly, Jamil, Jawhari, Maan, Najjar, Carole, Sobh, Hana, Abou-Jawdah, Yusuf
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.07.2014
• Subjects: Dot blot immunoassay; ELISA; Multiplexing; Potato virus S; Potato virus X; Potato virus Y; Solanum tuberosum; Tissue print immunoassay; Virus detection; Multiplexing; Tissue print immunoassay; Virus detection; Dot blot immunoassay; ELISA
• ISSN: 0261-2194; 1873-6904
• DOI: 10.1016/j.cropro.2014.03.007

Development of alternative serological techniques to ELISA for detection of potato viruses offers advantages for monitoring virus incidence and for seed potato certification systems. Several trials showed that multiplex tissue print immunoassay (TPIA) and dot blot immunoassay (DBIA) might represent fast, practical, and sensitive alternatives for the detection of: Potato leaf roll virus (PLRV), Potato virus S (PVS), Potato virus X (PVX) and Potato virus Y (PVY), from green and/or tuber tissues. In TPIA, the specific precipitation patterns in infected tissues of leaf petioles or stem cross sections, observed with each virus, allowed identification of the specific virus or mixed infections in a single multiplex assay. For detection of PVY in green tissues, DBIA was shown to be over 50 times more sensitive than ELISA. TPIA and ELISA from the tuber stem end or from eyes might be used for rapid detection of PVY and PVS in seed potato tubers without prior germination. PVS was evenly distributed in potato tuber tissue, while PVY was localized in the vascular tissue beneath the epidermis, with irregular distribution along the periphery of the potato tuber. For laboratories in developing countries lacking time and facilities for tests based on tuber germination, monitoring for PVS and PVY using TPIA in tuber tissue may be a suitable alternative to ELISA. •Multiplex TPIA and DBIA could replace ELISA for detection of some potato viruses.•Each virus gave a unique precipitation pattern enabling development of multiplex TPIA assay.•DBIA was shown to be more sensitive than ELISA when testing from potato leaves.•TPIA and ELISA could be used for PVY and PVS detection directly from non-germinating potato tuber.