Determination of lovastatin in human plasma using reverse-phase high-performance liquid chromatography with UV detection

The authors have developed a simple, rapid HPLC assay with ultraviolet (UV) detection for the analytical determination of lovastatin and its acid in human plasma for a concentration range of 100-5,000 ng/mL. Sample clean-up involved the use of C10 solid-phase extraction cartridges. Our limit of quan...

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Bibliographic Details
Published inTherapeutic drug monitoring Vol. 22; no. 6; p. 737
Main Authors Ye, L Y, Firby, P S, Moore, M J
Format Journal Article
LanguageEnglish
Published United States 01.12.2000
Subjects
Anticholesteremic Agents - blood
Calibration
Carboxylic Acids - blood
Chromatography, High Pressure Liquid - methods
Drug Monitoring - methods
Humans
Hydrolysis
Hydroxymethylglutaryl-CoA Reductase Inhibitors - blood
Lovastatin - blood
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Temperature
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Summary:The authors have developed a simple, rapid HPLC assay with ultraviolet (UV) detection for the analytical determination of lovastatin and its acid in human plasma for a concentration range of 100-5,000 ng/mL. Sample clean-up involved the use of C10 solid-phase extraction cartridges. Our limit of quantitation was 100 ng/mL. Standard curves were linear from 100 to 5,000 ng/mL, with a correlation coefficient (r2) of 0.999 +/- 0.0002. Stored samples were stable at -70 degrees C for up to 4 months prior to reversed-phase HPLC analysis. This assay was able to measure steady-state lovastatin concentration (Css) at the initial dose level in a phase I trial of lovastatin as a modulator of apoptosis.
ISSN:0163-4356
DOI:10.1097/00007691-200012000-00014