Studies on Human Porin XVIII: The Multicompartment Effector Ruthenium Red Reduces the Voltage Dependence of Human VDAC in Planar Lipid Bilayers

• Published in: Molecular genetics and metabolism Vol. 65; no. 3; pp. 246 - 249
• Main Authors: Siadat, Susan, Reymann, Susanne, Horn, Andrea, Thinnes, Friedrich P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.1998
• Subjects: channel regulation; Compound 48/80; eukaryotic porin; exocytosis; Humans; Ion Channel Gating - drug effects; Lipid Bilayers; Membrane Proteins - drug effects; Membrane Proteins - metabolism; p-Methoxy-N-methylphenethylamine - pharmacology; plasma membrane; Polyamines - pharmacology; Porins; ruthenium red; Ruthenium Red - pharmacology; VDAC; Voltage-Dependent Anion Channels; Compound 48/80; ruthenium red; plasma membrane; VDAC; channel regulation; exocytosis; eukaryotic porin
• ISSN: 1096-7192; 1096-7206
• DOI: 10.1006/mgme.1998.2764

The initial data on the effect of ruthenium red on mature human type-1 VDAC are presented. Highly enriched human type-1 porin in planar lipid bilayers shows lowered voltage-dependence whenever a commercially available ruthenium red preparation is applied. The hexavalent polycationic dye ruthenium red affects different functions in varying cell compartments. Concerning the plasma membrane of cells the actual data, together with our former measurements on the interaction of VDAC and the polycationic synthetic polyamine Compound 48/80, refer to a second VDAC opener, which is relevant for studies on the stimulation of exocytotic processes of different cell types.