Kluyveromyces lactis β-galactosidase crystallization using full-factorial experimental design

• Published in: Journal of molecular catalysis. B, Enzymatic Vol. 52; pp. 178 - 182
• Main Authors: Rodríguez, Ángel Pereira, Leiro, Rafael Fernández, Cerdán, M. Esperanza, González Siso, M. Isabel, Fernández, Manuel Becerra
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2008; Elsevier Science
• Subjects: Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Crystallization; Full-factorial design; Fundamental and applied biological sciences. Psychology; Kluyveromyces lactis; Methods. Procedures. Technologies; Purification; β-Galactosidase; Kluyveromyces lactis; Full-factorial design; Purification; β-Galactosidase; Crystallization; Ascomycota; Kluyveromyces marxianus var. lactis; Yeast; Enzyme; Fungi; Glycosylases; Experimental design; Glycosidases; Hydrolases; Factorial design
• ISSN: 1381-1177; 1873-3158
• DOI: 10.1016/j.molcatb.2007.11.013

Kluyveromyces lactis β-galactosidase is an enzyme with numerous applications in the environmental, food and biotechnological industries. Despite of its biotechnological interest, its three-dimensional structure has not yet been determined. The growth of suitable crystals is an essential step in the structure determination of a protein by X-ray crystallography. At present, crystals are mostly grown using trial-and-error procedures since their growth often depends on the combination of many different factors. Testing the influence on crystallization of even only a small number of these factors requires many experimental set-ups and large amounts of protein. In the present work, a full-factorial design has been used in order to find conditions for obtaining good-quality crystals of K. lactis β-galactosidase. With this full-factorial method protein crystals have been obtained.