Jellyfish skin polysaccharides: Extraction and inhibitory activity on macrophage-derived foam cell formation

• Published in: Carbohydrate polymers Vol. 106; pp. 393 - 402
• Main Authors: ZHANG, Hai-Lin, CUI, Shao-Hua, ZHA, Xue-Qiang, BANSAL, Vibha, LEI XUE, LI, Xiao-Long, RAN HAO, PAN, Li-Hua, LUO, Jian-Ping
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier 15.06.2014
• Subjects: 1-Butanol - pharmacology; Animals; Applied sciences; Atherosclerosis - genetics; Biological and medical sciences; Cardiovascular system; Cell Line; Chemical Precipitation; Chloroform - pharmacology; Cholesterol - analysis; Down-Regulation - drug effects; Drug Evaluation, Preclinical; Ethanol - pharmacology; Exact sciences and technology; Foam Cells - chemistry; Foam Cells - drug effects; Gene Expression Regulation - drug effects; Lipoproteins, LDL - pharmacology; Macrophages - chemistry; Macrophages - drug effects; Medical sciences; Mice; Molecular Weight; Natural polymers; Papain - pharmacology; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Real-Time Polymerase Chain Reaction; Scyphozoa - chemistry; Solvents - pharmacology; Spectrophotometry, Infrared; Starch and polysaccharides; Vascular wall; Tegument; Purification; Molecular weight distribution; Molecular structure; Animal origin; Foam cell; Response surface methodology; Experimental study; Solvent extraction; Deproteinization; In vitro; Biological activity; Optimization; Operating conditions; Rhopilema esculetum; Coelenterata; Jellyfish; Anti-astherosclerotic; Cnidaria; Atherosclerosis; Oside polymer; Invertebrata; Polysaccharide; Macrophage
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2014.01.041

In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP). The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h. Under these conditions, the JSP yield reached 1.007 mg/g. Papain (15 U/mL) in combination with Sevag reagent was beneficial in removing proteins from JSP. After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively. The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition. In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.