Immobilization of a recombinant strain producing glucose isomerase inside SiO₂-xerogel and properties of prepared biocatalysts

• Published in: Applied biochemistry and microbiology Vol. 47; no. 2; pp. 151 - 157
• Main Authors: Kovalenko, G. A, Perminova, L. V, Chuenko, T. V, Sapunova, L. I, Shlyakhotko, E. A, Lobanok, A. G
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.03.2011; SP MAIK Nauka/Interperiodica; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biotechnology; Catalysts; Cellular biology; E coli; Enzymes; Glucose; Life Sciences; Medical Microbiology; Microbiology; Substrate Conversion; Microbial Biomass; Apply Biochemistry; Fructose; Recombinant Strain
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683811020074

An original method of immobilization of non-growing microorganism cells inside xerogel of silicium dioxide containing insoluble hydroxyl compounds of cobalt(II) has been developed. A recombinant strain producing glucose isomerase has been constructed on the basis of Escherichia coli with the use of a gene of Arthrobacter nicotianae. It was revealed that glucose isomerase activity and stability of biocatalysts prepared on the basis of the recombinant E. coli strain was 3-5 times greater compared with the biocatalysts prepared with the use of the donor strain A. nicotianae. Under conditions of continuous hydrolysis of 3 M fructose at 62-65°C in a fixed bed reactor, time of half-inactivation of the biocatalysts prepared from the recombinant strain and A. nicotianae was ∼60 and ∼25 days, respectively.