Progress in Chemical Synthesis of Peptides and Proteins

• Published in: Transactions of Tianjin University Vol. 23; no. 5; pp. 401 - 419
• Main Authors: Hou, Wen, Zhang, Xiaohong, Liu, Chuan-Fa
• Format: Journal Article
• Language: English
• Published: Tianjin Tianjin University 01.09.2017; Springer Nature B.V; School of Life Sciences, Tianjin University, Tianjin 300072, China; School of Biological Sciences, Nanyang Technological University, Singapore 639798, Singapore%School of Biological Sciences, Nanyang Technological University, Singapore 639798, Singapore
• Subjects: Chemical synthesis; Engineering; Humanities and Social Sciences; ligation; ligation;Chemical; Mechanical Engineering; multidisciplinary; Peptide; Peptides; protein; Protein synthesis; Proteins; Review; Science; synthesis;Sequential; Sequential peptide ligation; Chemical protein synthesis; Peptide ligation
• ISSN: 1006-4982; 1995-8196
• DOI: 10.1007/s12209-017-0068-8

For the proteins that cannot be expressed exactly by cell expression technology (e.g., proteins with multiple posttranslational modifications or toxic proteins), chemical synthesis is an important substitute. Given the limited peptide length offered by solid-phase peptide synthesis invented by Professor Merrifield, peptide ligation plays a key role in long peptide or protein synthesis by ligating two small peptides to a long one. Moreover,high-molecularweight proteins must be synthesized using two or more peptide ligation steps, and sequential peptide ligation is such an efficient way. In this paper, we reviewed the development of chemical protein synthesis, including solid-phase peptide synthesis, chemical ligation, and sequential chemical ligation.