Activation of fibroblast proliferation by Werner's syndrome fibroblast-conditioned medium

• Published in: Journal of dermatological science Vol. 7; no. 3; pp. 210 - 216
• Main Authors: Hatamochi, Atsushi, Arakawa, Masami, Takeda, Koji, Ueki, Hiroaki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.06.1994
• Subjects: Adult; Cell Division - drug effects; Cell proliferation; Cells, Cultured; Collagen - metabolism; Conditioned medium; Culture Media, Conditioned - pharmacology; Female; Fibroblasts; Fibroblasts - pathology; Humans; Infant; Male; Middle Aged; Proteins - metabolism; Werner Syndrome - pathology; Werner's syndrome; Cell proliferation; PBS; DMEM; Werner's syndrome; Conditioned medium; FBS; Fibroblasts; PDL; WS
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/0923-1811(94)90097-3

The effects of Werner's syndrome (WS) fibroblast-conditioned medium on cell proliferation and collagen production of normal fibroblasts were studied using four WS fibroblast strains. The conditioned medium from the WS fibroblasts brought about activation of normal fibroblast proliferation, whereas, that from late passage normal fibroblasts and fibroblasts from aged donors' skin did not. Collagen and non-collagenous protein synthesis in normal fibroblasts were increased by the addition of conditioned medium from the WS fibroblasts, but the relative rates of collagen synthesis and non-collagenous protein synthesis were unaltered. These results suggest that activation of fibroblast proliferation by conditioned medium is one of the characteristics of WS fibroblasts.