Endcaps for Stabilizing Short DNA Duplexes

The syntheses of endcaps for covalently linking the 3′ and 5′ hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the e...

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Published inNucleosides, nucleotides & nucleic acids Vol. 22; no. 5-8; pp. 1635 - 1637
Main Authors Ng, Pei-Sze, Pingle, Maneesh R., Balasundarum, Ganesan, Friedman, Alan, Zu, Xiaolin, Bergstrom, Donald E.
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis Group 01.10.2003
Subjects
Amide
Base Sequence
DNA - chemistry
DNA duplexes
Drug Stability
Endcap
Models, Molecular
Nucleic Acid Conformation
Nucleic Acid Denaturation
Stabilization
Terthiophene
Thermodynamics
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Summary:The syntheses of endcaps for covalently linking the 3′ and 5′ hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by T m experiments on the self-complementary octamer.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1525-7770
1532-2335
DOI:10.1081/NCN-120023088