RNA AND N3′ → P5′ KISSING APTAMERS TARGETED TO THE TRANS-ACTIVATION RESPONSIVE (TAR) RNA OF THE HUMAN IMMUNODEFICIENCY VIRUS-1

We used in vitro selection to identify RNA aptamers able to selectively bind to the TAR RNA motif of HIV-1, an unperfect RNA hairpin involved in the transcription of the retroviral genome. We selected aptameric RNA hairpins giving rise to kissing complexes with TAR. The N3′ → P5′ phosphoramidate var...

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Published inNucleosides, nucleotides & nucleic acids Vol. 20; no. 4-7; pp. 441 - 449
Main Authors Darfeuille, Fabien, Cazenave, Christian, Gryaznov, Sergei, Ducongé, Frédéric, Di Primo, Carmelo, Toulmé, Jean-Jacques
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis Group 01.04.2001
Subjects
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
HIV Long Terminal Repeat - genetics
HIV-1 - genetics
Humans
Nucleic Acid Conformation
Oligonucleotides - chemical synthesis
Oligonucleotides - genetics
Oligonucleotides - pharmacology
Response Elements - genetics
RNA - genetics
RNA - metabolism
RNA-Binding Proteins - biosynthesis
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Substrate Specificity
Surface Plasmon Resonance
Transcriptional Activation
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Summary:We used in vitro selection to identify RNA aptamers able to selectively bind to the TAR RNA motif of HIV-1, an unperfect RNA hairpin involved in the transcription of the retroviral genome. We selected aptameric RNA hairpins giving rise to kissing complexes with TAR. The N3′ → P5′ phosphoramidate variant of the aptamer bind to TAR with a Kd in the low nanomolar range. However, only the RNA-RNA loop-loop complex is recognized by the Rop protein of E. coli which is specific for kissing complexes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1525-7770
1532-2335
DOI:10.1081/NCN-100002318