Detection of glycan-binding proteins using glycan-functionalized quantum dots and gold nanoparticles

Many pathogens can use their glycan-binding proteins (GBPs) to interact with glycan receptors on host cell surface. Here, a novel strategy is presented for the detection of GBPs based on fluorescence resonance energy transfer (FRET). Using mannose-functionalized quantum dots and mannose-functionaliz...

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Bibliographic Details
Published inJournal of carbohydrate chemistry Vol. 37; no. 4; pp. 199 - 209
Main Authors Zhang, Zhenxing, Qi, Xiaoxiao, Chai, Jinfeng, Wu, Peixing, Lv, Xun, Cheng, Shuihong, Li, Xuebing
Format Journal Article
LanguageEnglish
Published Philadelphia Taylor & Francis 04.05.2018
Taylor & Francis Ltd
Subjects
Binding
Concanavalin A
Energy transfer
Fluorescence
fluorescence resonance energy transfer
Glycan
glycan-binding protein
Gold
gold nanoparticles
Mannose
Nanoparticles
Pathogens
Proteins
Quantum dots
Receptors
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Summary:Many pathogens can use their glycan-binding proteins (GBPs) to interact with glycan receptors on host cell surface. Here, a novel strategy is presented for the detection of GBPs based on fluorescence resonance energy transfer (FRET). Using mannose-functionalized quantum dots and mannose-functionalized gold nanoparticles, a FRET assay system was developed and a mannose-specific GBP concanavalin A could be detected in 5 min with a detection limit of 3 nM. This result indicated the potential for rapid and sensitive biosensing of various kinds of GBPs and pathogens by changing the glycan structure on the surface of QDs and AuNPs.
ISSN:0732-8303
1532-2327
DOI:10.1080/07328303.2018.1451875