Formation of plantlets through somatic embryogeny in the Himalayan blue poppy, Meconopsis simplicifolia (Papaveraceae)

Exuberant and subculturable calli could be induced from only hypocotyl and leaf segments of ca 4-month-old seedlings of Meconopsis simplicifolia cultured on Murashige & Skoog's medium supplemented with 10(-6)M kinetin + 10(-5)M alpha-naphthalene acetic acid. Suspension cultures were initiat...

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Bibliographic Details
Published inPlant cell reports Vol. 9; no. 10; pp. 582 - 585
Main Authors Sulaiman, I.M, Rangaswamy, N.S, Babu, C.R
Format Journal Article
LanguageEnglish
Published Germany 01.02.1991
Subjects
callus
culture media
hypocotyls
leaves
Meconopsis
regenerative ability
somatic embryogenesis
tissue culture
India
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Summary:Exuberant and subculturable calli could be induced from only hypocotyl and leaf segments of ca 4-month-old seedlings of Meconopsis simplicifolia cultured on Murashige & Skoog's medium supplemented with 10(-6)M kinetin + 10(-5)M alpha-naphthalene acetic acid. Suspension cultures were initiated from the calli in a similar medium but with 10(-5)M 2,4-dichlorophenoxy acetic acid in place of alpha-naphthalene acetic acid. In ca 80% of the suspension cultures somatic embryos differentiated freely (80-85%) as well as on the surface of small clumps of tissue (15-20%). Somatic embryos that developed beyond heart-shaped stage were transferred to agar-solidified Murashige & Skoog's medium free of growth substances. When maintained in 10 h light and 14 h dark the somatic embryos developed into plantlets bearing cauline leaves. From seed sowing to raising normal plantlets via callus required 28 weeks; on average 80 plantlets were obtained from one explant in three passages.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00232337