The Effect of Ozone Dosage of Sunflower and Olive Oils on Their Biological Activities and Chemical Properties

• Published in: Ozone: science & engineering Vol. 46; no. 3; pp. 197 - 216
• Main Authors: Jacinto, Gabriel Serpa, Benvenutti, Larissa, Santin, José Roberto, de Freitas, Rilton Alves, Bella Cruz, Alexandre, Corrêa, Rogerio, Malheiros, Angela, Klein-Junior, Luiz Carlos, Bresolin, Tania Mari Bellé
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 03.05.2024; Taylor & Francis Ltd
• Subjects: Acidity; Analytical methods; Anti-inflammatory activity; Anti-inflammatory agents; antimicrobial activity; Biological activity; Biological properties; Calorimetry; Chemical properties; Chemicophysical properties; Chromatography; Differential scanning calorimetry; Dosage; Fungicidal activity; Fungicides; Gas chromatography; Helianthus; Inflammation; Infrared spectroscopy; Iodine; Magnetic resonance; Nitric oxide; NMR; Nuclear magnetic resonance; Oil; Olive oil; ozonated oil; Ozonation; Ozone; Ozonization; Physicochemical processes; Physicochemical properties; quality control; Standardization; Sunflower oil; Thermogravimetry; Vegetable oils; Vegetables; Viscosity
• ISSN: 0191-9512; 1547-6545
• DOI: 10.1080/01919512.2023.2266734

The ozonation process of vegetable oils is commonly monitored by peroxide value (PV). However, PV methodology has been adapted from vegetable oils, with protocol divergences. In addition, few studies are based on the minimum level of ozonation to ensure biological activity. This study aimed to optimize the PV methodology for sunflower oil with different ozone dosage aiming to discriminate these samples. Also, to establish the relationship between its physicochemical properties with the biological activity. The optimized PV method was also applied to ozonated olive oil, as well as the other analytical techniques of iodine value (IV), acidity value (AV), density, viscosity, gas chromatography, infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), differential scanning calorimetry and thermogravimetry (DSC and TG). The PV method discriminated the ozonation level of the samples. Both ozonated sunflower and olive oils need to reach an intermediary ozone dosage to perform the in vitro bactericidal and fungicidal activity (MIC ≤2.5%), and anti-inflammatory activity (inhibition of nitric oxide release in Raw cells). These results point to the importance of standardizing the ozone dosage for each oil and the analytical methodologies used in its characterization.