Surface decontamination of plant tissue explants with chlorine dioxide gas

• Published in: In vitro cellular & developmental biology. Plant Vol. 51; no. 2; pp. 214 - 219
• Main Authors: Bhawana, Stubblefield, Jeannie M., Newsome, Anthony L., Cahoon, A. Bruce
• Format: Journal Article
• Language: English
• Published: Boston Springer 01.04.2015; Springer US; Springer Nature B.V
• Subjects: Bacteria; Biomedical and Life Sciences; Cell Biology; Developmental Biology; Disinfection & disinfectants; Life Sciences; Microorganisms; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant propagation; Plant Sciences; PROTOCOLS/METHODS; Sodium; Studies; Cauliflower; Micropropagation; Plant tissue culture; Chlorine dioxide gas
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-014-9659-4

The surfaces of plant tissues readily harbor bacteria, fungi, and spores making their preparation and growth by aseptic micropropagation difficult. Recent innovations in chlorine dioxide (ClO₂) gas production now allow for the generation of small amounts of the gas to meet specific needs. This report demonstrates the efficacy of ClO₂ gas in the preparation of plant tissues for micropropagation. Cauliflower curd was surface decontaminated with 1500, 600, 300, and 150 ppm of gas for 30, 60, 180, and 360 min, and the presence of tissue surfaces for microbiota were assayed. The use of ClO₂ gas in this system decontaminated cauliflower curd surfaces at rates equal to bleach treatment, but the ClO₂-treated tissues grew more quickly and were healthier than the bleach-treated cauliflower curd samples. Chlorine dioxide gas potentially represents an easy means to surface decontaminate plant tissues intended for micropropagation that, when properly used, does not pose a risk to human health.