A transcriptional atlas of the silk gland in Antheraea pernyi revealed by IsoSeq

• Published in: Journal of Asia-Pacific entomology Vol. 26; no. 2; pp. 102043 - 8
• Main Authors: Duan, Jianping, Li, Shanshan, Zhang, Zhengtian, Yao, Lunguang, Yang, Xinfeng, Ma, Sanyuan, Duan, Nini, Wang, Jiazhen, Zhu, Xuwei, Zhao, Ping
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2023; 한국응용곤충학회
• Subjects: Antheraea pernyi; Full-length transcriptome; Genic annotation; Silk gland; Transcription factor; 농학; AA; lncRNA; AD; FL; IR; ICE; PID; MEE; Full-length transcriptome; Genic annotation; ES; AS; APA; TF; CCS; Silk gland; nFL; Antheraea pernyi; FLNC; Transcription factor
• ISSN: 1226-8615; 1876-7990
• DOI: 10.1016/j.aspen.2023.102043

[Display omitted] •A full-length transcriptional landscape of the silk gland in Antheraea pernyi was completed.•7,568 genic loci were covered by 12,572 FL isoforms from the A. pernyi silk gland.•1,121 lncRNAs and 871 TFs were first identified in the A. pernyi silk gland.•Some TF families underwent expansion or contraction of their members. Silk fibers spun by the silk gland of Antheraea pernyi have many unique properties and are of great value in genetic improvement and non-traditional applications. However, the complete transcriptional landscape and accurate genic annotation of the silk gland are yet to be conducted, which limits related studies on this organ. In this study, isoform sequencing revealed the full-length transcriptome of the A. pernyi silk gland, producing 12,572 high-confidence isoforms from 7,658 genes, among which more than 40 % of genes have not yet been annotated in the reference genome. Moreover, approximately 9 % of isoforms are computationally identified as long non-coding RNAs (lncRNAs). Up to 1,492 alternative splicing (AS) and 3,068 alternative polyadenylation (APA) events were revealed within this transcriptome. In addition, 2,569 putative transcription factors (TFs) belonging to 68 different families were first identified in A. pernyi genome, including 871 TFs in silk gland, and some TF families have undergone expansion or contraction. This study significantly improve our knowledge of the genes expressed in the silk gland of A. pernyi and provide a valuable resource for the in-depth study of silk protein synthesis and spinning, genetic improvement, and non-traditional applications in A. pernyi.