Method development and optimisation for analysis of pesticide residues in biological matrices by modified QuEChERS and gas chromatography-triple quadrupole mass spectrometry

A rapid and precise modified QuEChERS method for extraction was developed for simultaneous quantitation of multi-class pesticide residues in human biological matrices, viz. blood, serum and urine. In the modified QuEChERS method, targeted analytes in biological sample were extracted using acidified...

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Bibliographic Details
Published inInternational journal of environmental analytical chemistry Vol. 104; no. 13; pp. 3065 - 3079
Main Authors Kottadiyil, Divya, Thasale, Rupal, Deore, Shital, Sivaperumal, P.
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 20.10.2024
Taylor & Francis LLC
Subjects
Acetates
Acetic acid
Acetonitrile
Acidification
Biological analysis
Biological properties
Biological samples
Blood
Ethyl acetate
Gas chromatography
GC-MS/MS
Mass spectrometry
Mass spectroscopy
matrix effect
modified QuEChERS
Parameter modification
Pesticide residues
Pesticides
Quadrupoles
Quantitation
Residues
serum
Solid phases
urine
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Summary:A rapid and precise modified QuEChERS method for extraction was developed for simultaneous quantitation of multi-class pesticide residues in human biological matrices, viz. blood, serum and urine. In the modified QuEChERS method, targeted analytes in biological sample were extracted using acidified solution of acetonitrile and ethyl acetate along with anhydrous MgSO 4 . The collected aqueous layer was evaporated and dispersive solid-phase extraction clean-up was performed. The pesticide residues were assessed using gas chromatography-tandem mass spectrometry with multiple reaction monitoring mode. The developed method was validated as per European Union SANTE/12682/2019 guidelines and the evaluated method validation parameters, viz. linearity, limit of detection, limit of quantitation, accuracy, %relative standard deviation and matrix effect were found to be satisfactory. The proposed method is easy, comprehensible and efficient for simultaneous analysis of diverse range of pesticide residues within similar class. Hence, the present solitary method can be used for systematic analysis of biological samples.
ISSN:0306-7319
1029-0397
DOI:10.1080/03067319.2022.2076228