Automated method for the solid phase extraction of tetracyclines in wastewater followed by fluorimetric determination

• Published in: Talanta (Oxford) Vol. 270; p. 125544
• Main Authors: Vargas-Muñoz, M A, Boudenne, Jean-Luc, Coulomb, Bruno, Robert-Peillard, Fabien, Palacio, Edwin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier 01.04.2024
• Subjects: Analytical chemistry; Chemical Sciences; Automation; MSFIA; Fluorimetry; Tetracyclines; Solid-phase extraction; Wastewater analysis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2023.125544

This work presents a multisyringe flow injection analysis (MSFIA) system for the automatic extraction and determination of tetracyclines in wastewater samples. The sample was adjusted with Na EDTA buffer before solid-phase extraction with an Oasis HLB column used for the analyte preconcentration. The europium (Eu )-based and citrate-mediated method (using Tris-HCl buffer) was selected for the fluorimetric analysis (λ  = 400/612 nm). For fluorescence detection, a low-cost system consisting of an USB 2000 CCD detector and a 3D-printed support that holds a LED light source was used. Under optimized conditions, the proposed method provided low limits of detection (9.4 μg L ) and quantification (31 μg L ), and good values for intra-day (<4 %) and inter-day precisions (<6 %). Recoveries of spiked TCs in wastewater samples ranged from 87 to 106 %. The results of this work were in good agreement with the measurements obtained by liquid chromatography coupled to a fluorescence detector.