Controlled expression of tagged proteins in Drosophila using a new modular P-element vector system

• Published in: Molecular & general genetics Vol. 262; no. 6; pp. 916 - 920
• Main Authors: Schotta, G, Reuter, G
• Format: Journal Article
• Language: English
• Published: Germany 01.01.2000
• Subjects: animal proteins; Animals; Animals, Genetically Modified; Base Sequence; DNA Primers - genetics; Drosophila melanogaster; Drosophila melanogaster - genetics; Gene Expression; genes; genetic transformation; Genetic Vectors; green fluorescent protein; Green Fluorescent Proteins; heterochromatin; Heterochromatin - genetics; Luminescent Proteins - genetics; P-element; plasmid vectors; polytene chromosomes; promoter regions; Promoter Regions, Genetic; protein tags; Recombinant Fusion Proteins - genetics; reporter genes; Scyphozoa; su(var)3-9 gene; Transformation, Genetic; transposon P; transposons
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/PL00008659

We have developed a new modular vector system that facilitates the combination of various DNA fragments as functional modules for P element-mediated transformation of Drosophila melanogaster. The basic vector pP¿GS¿ contains unique sites for 17 restriction enzymes, including three 8-bp cutters, that allow one to combine various promoter elements, cDNAs and genomic DNA fragments, as well as protein tags and selectable marker genes, for a wide spectrum of transgene analyses. With this new vector system we analysed the chromosomal distribution of the Drosophila SU(VAR)3-9 protein tagged with EGFP, using hsp70-cDNA and genomic Su(var)3-9 constructs. We found preferential association of the tagged SU(VAR)3-9 with centric heterochromatin.