In vitro culture of rudimentary embryos of Ilex paraguariensis: responses to exogenous cytokinins

• Published in: Journal of plant growth regulation Vol. 17; no. 2; pp. 101 - 105
• Main Authors: Sansberro, P.A. (Facultad de Ciencias Agrarias, Corrientes, Argentina.), Rey, H.Y, Mroginski, L.A, Collavino, M.M
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 1998; Springer Nature B.V
• Subjects: 6-BENZILADENINA; 6-BENZYLADENINE; ADENINA; ADENINE; ANATOMIA DE LA PLANTA; ANATOMIE VEGETALE; BEVERAGE CROPS; Biological and medical sciences; Chemical agents; CULTIVO DE EMBRIONES; CULTURE D'EMBRYON; CULTURE MEDIA; DEVELOPMENTAL STAGES; DOSAGE EFFECTS; EFECTOS DE DOSIFICACION; EFFET DOSE; EMBRIONES VEGETALES; EMBRYO CULTURE; EMBRYON VEGETAL; Embryos; ETAPAS DE DESARROLLO; Fundamental and applied biological sciences. Psychology; ILEX PARAGUARIENSIS; ISOPENTENYLADENINE; KINETIN; KINETINE; MATE (TE); MATE (TEA); MATE (THE); MEDIO DE CULTIVO; MICROPROPAGACION; MICROPROPAGATION; MILIEU DE CULTURE; MORFOGENESIS; MORPHOGENESE; MORPHOGENESIS; MURASHIGE AND SKOOD MEDIUM; PLANT ANATOMY; PLANT EMBRYOS; Plant physiology and development; PLANTAS PARA BEBIDAS; PLANTE A BOISSON; QUINETINA; Seedlings; STADE DE DEVELOPPEMENT; THIDIAZURON; TIDIAZURON; Vegetative apparatus, growth and morphogenesis. Senescence; ZEATIN; ZEATINA; ZEATINE; Adenine derivatives; Adenine(N; Exogenous; Purine derivatives; Embryo; Growth; Tissue culture; Stimulant plant; Cytokinin; In vitro; Dose activity relation; Aquifoliaceae; Morphogenesis; Dicotyledones; Angiospermae; Plant growth substance; Ureas; Kinetin; Spermatophyta; benzyl
• ISSN: 0721-7595; 1435-8107
• DOI: 10.1007/PL00007014

The effects of benzyladenine (BAP), kinetin (KIN), zeatin (ZEA), isopentenyladenine (2iP), and thidiazuron (TDZ) were studied on in vitro growth of rudimentary embryos of Ilex paraguariensis St. Hil. Heart stage zygotic embryos were removed from seeds of immature, light green fruits and cultured aseptically on quarter-strength Murashige and Skoog medium containing 3% sucrose, 0.65% agar, and supplemented with or without three concentrations of BAP, KIN, ZEA, 2iP, or TDZ. Cultures were incubated in darkness at 27 +/- 2 degrees C. Media containing 4.4 x 10(-6) M BAP, 4.6 x 10(-6) M KIN, or 4.9 x 10(-6) M 2iP were totally ineffective in inducing embryo growth after culture for 28 days. However, lower concentrations of these compounds (4.4 x 10(-8) M BAP, 4.6 x 10(-8) M KIN, 4.5 x 10(-8) M ZEA, or 4.9 x 10(-8) M 2iP) promoted embryo growth. TDZ at 9.9 x 10(-9) M, 9.9 x 10(-8) M, or 9.9 x 10(-7) M induced embryo growth at similar rates. The maximum percentage of embryos converted to seedlings was achieved when the medium was supplemented with 4.5 x 10(-7) M ZEA