Acmella radicans var. radicans: In vitro Culture Establishment and Alkamide Content

• Published in: In vitro cellular & developmental biology. Plant Vol. 39; no. 1; pp. 37 - 41
• Main Authors: P. Rios-Chavez, E. Ramirez-Chavez, C. Armenta-Salinas, Molina-Torres, J.
• Format: Journal Article
• Language: English
• Published: Wallingford CABI Publishing 01.01.2003; Cambridge University Press; Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Amides; Biological and medical sciences; Economic plant physiology; Flasks; Flowers; Fundamental and applied biological sciences. Psychology; In vitro culture; Leaves; Metabolic Engineering/Secondary Metabolism; Plant biochemistry; Plant physiology and development; Plant propagation; Plant roots; Plantlets; Plants; Stems; Tissue cultures, protoplasts; Node; Tissue culture; Explant; Compositae; Microplant; Internode; Dicotyledones; Angiospermae; N-isobutyl-decamide; Secondary metabolism; Culture medium; Transplanting; single-node; N-isobutyl-2E-decamonoenamide; Micropropagation; Plant leaf; Regeneration; NMR; affinin; Asteraceae; Spermatophyta; Organic amide; Biological accumulation; hioactive metabolite; GC/MS
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1079/IVP2002354

Acmella radicans var. radicans propagation was established in vitro. This plant belongs to the Asteraceae from which some species are known for their insecticide, fungicide and antibacterial activity. The complete Murashige and Skoog (MS) medium was the best in assisting seed germination. In order to obtain shoots in vitro, a complete MS medium and half-strength MS medium were assayed with explants from leaves, nodes, and internodes. The best medium for shoot production was the half-strength MS medium with no addition of plant growth regulators, and the highest shoot propagation was from single-node explants. Regeneration of roots on shoot explants in the medium was obtained without the addition of growth regulators. Of the plantlets that were acclimated, 90% of them were obtained from rooted shoots with completely expanded leaves. The alkamide content was evaluated for each tissue and the higher concentration was observed in flower heads. The main alkamides present in the leaves and the flower heads were N-(2-phenylethyl)-2Z,4E-octadienamide and 3-phenyl-N-(2-phenylethyl)-2-propenamide. This study describes the methodology for the establishment and propagation of Acmella radicans in vitro and the evaluation of different tissue alkamide contents in vitro and in the field.