Characterization of the bioactive metabolite from a plant growth promoting rhizobacteria Pseudomonas aeruginosa VRKK1 and exploitation of antibacterial behaviour against Xanthomonas campestris a causative agent of bacterial blight disease in cowpea

• Published in: Archiv für Phytopathologie und Pflanzenschutz Vol. 55; no. 7; pp. 797 - 814
• Main Authors: Kanthaiah, Kannan, Velu, Rajesh Kannan
• Format: Journal Article
• Language: English; German
• Published: Abingdon Taylor & Francis 11.05.2022; Taylor & Francis Ltd
• Subjects: Antibacterial activity; biocontrol agent; Biological activity; biometabolite; Blight; Chloroform; Cowpea; Cowpeas; Exploitation; Gene sequencing; Metabolites; MIC; octadecanoic acid 2-oxo methyl ester; PGPR; Plant bacterial diseases; Plant diseases; Plant growth; Pseudomonas aeruginosa; Rhizosphere; rRNA 16S; Vigna unguiculata; Xanthomonas campestris
• ISSN: 0323-5408; 1477-2906
• DOI: 10.1080/03235408.2018.1557883

A plant growth-promoting rhizobacterial strain B6 was isolated from a Cowpea rhizosphere soil in the Vellodu village of Dindugul district, Tamil Nadu, India. It could be identified as Pseudomonas aeruginosa VRKK1 through biochemical and 16S rDNA gene sequencing. The plant growth promoting behaviour already exhibited by this strain, further its bioactive metabolite extracted using chloroform. Crude extract and column purification study reveals that the idea of using this biometabolite against Xanthomonas campestris. F4 and F5 fractions showed 41 and 40 mm zone of inhibition which confirmed the broad spectrum antibacterial activity. Further this biometabolite analytically characterized and found to be as octadecanoic acid 2-oxo methyl ester. The identified biometabolite checked for the reduction of bacterial blight disease in cowpea with the disease severity percentage of 40.24% compared to control and induced plants.