Screening and Production of Industrially Relevant Enzymes by Bacillus paranthracis Strain MHDS3, a Potential Probiotic

The digestive process and intestinal protein absorption are influenced by a variety of factors. Due to their numerous health advantages, including potential favorable effects on protein digestion and absorption, probiotics have gained increased attention in recent years. Probiotics can control the i...

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Published inFermentation (Basel) Vol. 9; no. 11; p. 938
Main Authors Tshisikhawe, Musundwa Locardia, Diale, Mamonokane Olga, Abrahams, Adrian Mark, Serepa-Dlamini, Mahloro Hope
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.11.2023
Subjects
Bacillus
Bacillus paranthracis
Bacteria
Carbon
Cellulase
Cellulose
digestive enzymes
Enzymatic activity
enzyme activity
Enzymes
Epithelium
Fermentation
Gene clusters
Genomes
Glycerol
Immune system
Intestinal microflora
Intestine
Iodine
Metabolism
Microorganisms
Nitrogen
Pellaea calomelanos
Probiotics
Proteolysis
Quantitative analysis
Salinity
submerged fermentation
Temperature
Toxicity
Yeast
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Summary:The digestive process and intestinal protein absorption are influenced by a variety of factors. Due to their numerous health advantages, including potential favorable effects on protein digestion and absorption, probiotics have gained increased attention in recent years. Probiotics can control the intestinal microflora, which in turn affects the intestinal bacteria responsible for proteolysis. Additionally, certain probiotics can release exoenzymes that aid in the digestion of proteins and others can stimulate the host’s digestive protease and peptidase activity. By boosting transport and enhancing the epithelium’s capacity for absorption, probiotics can also improve the absorption of tiny peptides and amino acids as well as lessen detrimental protein fermentation, which lowers the toxicity of metabolites. The present study explored the production of enzymes by Bacillus paranthracis strain MHDS3, a probiotic candidate isolated from Pellaea calomelanos. Bacillus paranthracis displayed enzyme activities of amylase (31,788.59 IU), cellulase (4487.486 IU), and pectinase (13.98986 IU) through submerged fermentation. The CAZyme analysis of B. paranthracis revealed 16 CAZyme gene clusters associated with cellulose, amylase, and pectinase activity. Thus, B. paranthracis is a promising probiotic strain that can produce enzymes with biotechnological applications.
ISSN:2311-5637
2311-5637
DOI:10.3390/fermentation9110938