INHIBITION OF IN VITRO PRE-mRNA SPLICING IN S. CEREVISIAE BY BRANCHED OLIGONUCLEOTIDES

A series of V- and Y-shaped nucleic acids, related to the splicing intermediates derived from S. cerevisiae actin pre-mRNA, were prepared. The effects of such branched nucleic acids (bNAs) on the efficiency of in vitro pre-mRNA splicing in yeast were studied. The exogenous bNAs each effect the effic...

Full description

Saved in:
Bibliographic Details
Published inNucleosides, nucleotides & nucleic acids Vol. 20; no. 4-7; pp. 873 - 877
Main Authors Carriero, S., Braich, R. S., Hudson, R. H. E., Anglin, D., Friesen, J. D., Damha, M. J.
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis Group 01.04.2001
Subjects
Oligonucleotides - genetics
Oligonucleotides - pharmacology
RNA Precursors - antagonists & inhibitors
RNA Precursors - metabolism
RNA Splicing - drug effects
RNA, Messenger - antagonists & inhibitors
RNA, Messenger - metabolism
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Online AccessGet full text

Cover

More Information
Summary:A series of V- and Y-shaped nucleic acids, related to the splicing intermediates derived from S. cerevisiae actin pre-mRNA, were prepared. The effects of such branched nucleic acids (bNAs) on the efficiency of in vitro pre-mRNA splicing in yeast were studied. The exogenous bNAs each effect the efficiency of splicing, yet to different degrees, depending on the sugar composition and topology of the molecules. Y-shaped RNAs inhibited the formation of mRNA (i.e. RNA splicing) to the greatest extent.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1525-7770
1532-2335
DOI:10.1081/NCN-100002449